I’m Elizabeth R. Delos Santos, student of Philippine Normal University. I’m currently taking-up Bachelor in Chemistry for teachers and I’m now on my fourth year in this course. I’m hoping to graduate on March 2013 with my 21 classmates.
The fierce and sophisticated president. A strong woman who outrageously goes off her limits for the class. - From IV-BSCT
Bioassay is an assay designed to analyze any compound by use of a suitable biological system like animals, tissues, microbes etc.
It is defined as estimation or determination of concentration or potency of a physical, chemical or biological substance (agent) by means of measuring and comparing the magnitude of the response of the test with that of standard over a suitable biological system under standard set of conditions.
The bioassay compares the test sample with a same Internationally applicable standard substance. It determines the quantity of test sample required to produce an equivalent biological response to that of standard substance.
Standard samples are accepted by expert committee at international level and they represent fixed units of activity.
The Importance of Bioassay 1. They not only help to determine the concentration but also the potency of the sample. 2.It is especially used to standardize drugs, vaccines, toxins or poisons, disinfectants, antiseptics. 3. These also help determine the specificity of a compound to be used. 4. Certain complex compounds like Vitamin B-12which can't be analysed by simple assay techniques can be effectively estimatedby Bioassays.
Bioassay Systems and Techniques
1. In Vivo Technique These techniques employ a living animal recommended for the purpose of assay. The techniques aims to study the biological effect or response of the compound under screening in a living system directly.
2. In Vitro Technique These techniques employ a cell culture of recommended biological system to study the effect of compound under standard condition not similar to that of living environment. Here the cell culture survives by utilization of the nutrition in the media.
3. Ex Vivo Technique These techniques employ a tissue or cells of recommended living system to study the effect of compound under test in suitable conditions within the stipulated time of organ survival outside the body.